48 48 access array custom (fluidigm)

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fluidigm 48 48 access array custom
48 48 Access Array Custom, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48+48+access+array+custom/Access+Array/pmc06551272-103-47-46
Average 93 stars, based on 843 article reviews

48 48 access array custom - by Bioz Stars, 2026-10

93/100 stars

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Multiplex Assay:

Article Title: DNA-PKc deficiency drives pre-malignant transformation by reducing DNA repair capacity in concert with reprogramming the epigenome in human bronchial epithelial cells
Article Snippet: .. These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension method developed [ 26 ] the TruSight (Illumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung cancer related mutations . .. DNA samples from HBEC parent and transformed lines were used for the different sample preparations for the libraries and next generation sequencing was done on the Illumina MiSeq .

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Genetic analysis Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Polymerase Chain Reaction:

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Amplification:

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Next-Generation Sequencing:

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Mutagenesis:

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Sequencing:

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

Variant Assay:

Article Title: Common Cancer-Driver Mutations and their Association with Abnormally Methylated Genes in Lung Adenocarcinoma from Never-Smokers
Article Snippet: .. Targeted NGS was applied to screen a panel of 20 genes selected based on two recent publications that 1) identified frequent mutation of these genes in lung adenocarcinoma and 2) nominated the genes as drivers of lung adenocarcinoma because of the cancer-driver properties of the mutations [ 29 , 41 ], Mutation analysis was performed at the Emory Integrated Genomics Core and Biostatistics and Bioinformatics Shared resource of Winship Cancer Institute of Emory University using a combination of multiple mutation assays each as described in detail [ 42 , 43 ], These assays include the SNaPshot (Life Technologies, Grand Island, NY) multiplex PCR with single base primer extension, [ 44 ] the TruSight (lllumina Inc, San Diego, CA) tumor target amplicon-based library preparation kit, the HaloPlex Cancer NGS (Agilent Technologies, Santa Clara, CA), and the Fluidigm 48.48 Access Array custom designed for lung adenocarcinoma related mutations as described [ 43 ], DNA samples from lung adenocarcinoma tumor-normal pairs were used for the different sample preparations (SNaPshot, TruSight tumor, Access Array, and HaloPlex probe capture libraries) and NGS was completed using the lllumina MiSeq as described [ 42 , 43 ], Sequence alignment, index and primer trimming, and variant calls were performed using onboard MiSeq software. ..

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